| Ticker | Status | Jurisdiction | Filing Date | CP Start | CP End | CP Loss | Deadline |
|---|
| Ticker | Case Name | Status | CP Start | CP End | Deadline | Settlement Amt |
|---|
| Ticker | Name | Date | Analyst Firm | Up/Down | Target ($) | Rating Change | Rating Current |
|---|
Early access of novel technology that enables preparation of thousands of samples at low cost through self-normalization, enhanced multiplexing, and streamlined workflow
Innovative solution positioned to drive NGS adoption in AgBio
Twist Flex Prep UHT Kit and additional NGS tools to be highlighted at AGBT General Meeting
Twist Bioscience Corporation (NASDAQ:TWST), a company enabling customers to succeed through its offering of high-quality synthetic DNA using its silicon platform, today announced technology early access of the Twist Flex Prep Ultra High-Throughput (UHT) Kit, which aims to enable unparalleled throughput at a fraction of the cost of existing on market solutions, accelerating NGS' microarray conversion and adoption in agricultural genomics.
Built upon enzymatic fragmentation library preparation, the Twist Flex Prep UHT Kit utilizes novel chemistry that accepts and self-normalizes a wide range of sample input amounts through a new proprietary Normalization By Ligation (NBL) technology. This is crucial for researchers in AgBio who sequence thousands of samples of varying inputs and removes the need to manually normalize each one, saving significant time and costs. The kit also enables enhanced multiplexing by pooling samples early in the workflow so that researchers can process more samples without additional capital investment. The enhanced multiplexing further limits hands on time and saves researchers costs of reagents in downstream purification and indexing steps. In addition, pooling samples significantly reduces cost and plastic waste from pipette tips and plates in high-throughput labs.
"In agricultural genomics, researchers often test thousands of samples at once, relying heavily on automation to support large scale testing. Many researchers continue to perform traditional microarray based workflows, as overall higher cost per sample for NGS based workflow has hindered adoption," said Emily M. Leproust, Ph.D., CEO and co-founder of Twist Bioscience. "With the technology early access of our Twist Flex Prep UHT Kit, researchers will soon have access to a scalable, streamlined, and most importantly, cost effective NGS workflows for their various samples and research areas, and overcome the challenges that have caused slow adoption of NGS in the AgBio space. This ultra high-throughput solution could enable them to push new boundaries in breeding, trait selection, and other high-throughput applications, with high data quality while saving costs."
Twist Flex Prep UHT Kit Workflow
The Twist Flex Prep UHT Kit workflow is purpose-built to streamline and massively scale up Twist's established enzymatic fragmentation methods. The new NBL technology eliminates the need to measure quantity of each DNA sample and to perform time consuming concentration adjustment prior to library preparation, saving significant cost per sample. Following fragmentation and adapter ligation of each sample in a plate, up to 48 samples are pooled together in a single tube. This pooling strategy results in a meaningful reduction in both volumes and costs of reagents required for subsequent beads purification and amplification steps. Libraries prepared from this kit can be leveraged to perform standard whole genome sequencing (WGS), target enrichment, or low-pass WGS with enrichment.
By combining up to 48 unique adapters from the Twist UHT Flex Prep Kit with Twist's current high throughput Unique Dual Indexing (UDI) system, theoretical sample multiplexing can top more than 140,000. All plate and tube formats are optimized to ensure compatibility with key automation systems, a critical consideration for labs looking to streamline workflows and reduce hands-on time.
Presence at Advances in Genome Biology and Technology (AGBT) General Meeting
Twist will highlight its NGS tools in posters and presentations at the AGBT General Meeting taking place in Orlando, FL February 5-8, 2024. Details are as follows:
Poster: Twist Pan-Cancer Reference Standards V2: Enhanced Precision and Reduced Errors in ctDNA Analysis
Time: Tuesday, February 6, 1:30-3:30 PM ET
Presentation: Unveiling Next-Gen Solutions for Precision Diagnostics and Beyond
Speaker: Emily M. Leproust, CEO and co-founder of Twist Bioscience
Time: Wednesday, February 7, 3:00-3:20 PM PT
Location: Palms Ballroom 2 & 3
Poster: Development of a high-throughput NGS library preparation workflow with normalization adapters and in-line barcode technology
Time: Wednesday, February 7, 4:45-6:10 PM ET
Posted In: TWST
